Microscopy from Scientific Research

Bright-field microscopy images showing the absence and presence of protein condensates at different polyU concentrations and constant protein concentration after 30 min of incubation. Accordingly, we delineate three different regimes: (1) condensation driven by homotypic interactions; (2) condensation driven by both homotypic and heterotypic proteinRNA interactions; and (3) re-entrant phase and absence of condensation. The regimes are colour-coded in all figures by purple, blue and green colours, respectively. Bright-field imaging was always performed in parallel with turbidity measurements to confirm the presence of condensates, and was therefore repeated for two different protein preparations.

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