Microscopy from Scientific Research

Re-scan confocal microscopy images at different time points during hnRNPA1A aggregation mediated by condensation. The protein bulk concentration was 10 M, and this concentration was used for all the following experiments unless otherwise stated. The top row shows the fluorescence intensity of ThT, reporter for amyloid fibrils. The middle row shows the fluorescence intensity of Atto 647N hnRNPA1A. The bottom row shows an overlay of the ThT and Atto 647N hnRNPA1A fluorescence intensities. Already after 2 h, there is a change in the ThT fluorescence intensity at the interface of the condensates. At later time points, it is possible to notice fibrils emerging from the condensates. This experiment was repeated with at least three protein batches. We always observed a higher ThT signal at the interface of the condensates, but the timescale of fibril formation varied by 3-4 h.

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