Microscopy from Scientific Research

FDLD imaging reveals a preferential orientation of ThT, indicating that fibrils align parallel to the interface of the condensates. Following an additional 24 h of incubation, the fibrils continue to grow normally towards the interface. Vertical and horizontal refer to the polarization of the excitation beam. FDLD values are colour-coded in the image from yellow (vertically oriented) to blue (horizontally oriented). Grey values indicate no preferential orientation. The schematic diagram below the microscopy images shows how fibrils and proteins may be oriented with respect to the interface of the condensates. This experiment was independently conducted twice, with each run comprising two technical replicates, using the same protein batch.

DOI