(right) FRAP experiments on condensates within 30 min of incubation, showing ~98% signal recovery. Data points represent the mean, and error bars show standard deviations of measurements from nine individual condensates from three technical replicates ( n = 9). The experiment was repeated for two different protein batches yielding similar results. The hnRNPA1A concentration was 20 M, and the buffer composition was 20 mM Tris, pH 7.5 and 2 mM -mercaptoethanol. This buffer was used for all the following experiments unless otherwise stated. (left) Representative confocal microscopy images before and after photobleaching.
