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Comparison of IgG-dextran binding by cells expressing V(D)J mutated IgM from P1 to P4 with cells expressing membrane IgM corresponding to two clonally related IGHV1-69/IGKV3-20 RF autoantibodies with known affinities (TJ4 and TJ5). The IgG-dextran-binding threshold determined by visual inspection is shown by the dotted black arrow.
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