Binding of empty dextran (top row), HCV E2 H77 dextran (second row), and HCV E2 UNK3a.13.6 dextran E2 dextran (third row) and IgG dextran (bottom row) to transfected HEK293 cells expressing membrane IgM corresponding to the mutated anti-HCV E2 antibody AR3A (far left column), the unmutated ancestors of two anti-HCV E2 antibodies (B04 and H04), the unmutated ancestors of patients P1-P4, or a mutated IgM RF cryoglobulin from a Sjogren’s syndrome-cryovas patient, TJ4 (far right).
