Representative Ca 2+ oscillations recorded in YC3.6-expressing lines of WT, cngc15a GOF and cngc15c GOF before and after addition of Nod factor (NF) (10 -8 M). The traces show the ratio of yellow fluorescent protein (YFP)/cyan fluorescent protein (CFP) fluorescence in arbitrary units (a.u.). Stars indicate spontaneous Ca 2+ oscillations, and the numbers of plants responding over the total number of plants recorded are indicated on the right. Scale bars, 10 min