Clonal trees depicting the acquisition of individual V(D)J mutations by manual nearest neighbor analysis. Gray circles indicate inferred unmutated ancestor (ancestor) and inferred intermediate cells. Colored circles denote one or more sequenced cells sharing the identical immunoglobulin nucleotide sequence, with the size of the circle proportional to the number of cells (cell number indicated in center). Red asterisk or hash denotes one or more cells identified with the indicated driver mutation. Length of branches corresponds to the number of V(D)J mutations (see scale): solid lines, number of replacement mutations; dotted lines, number of silent mutations. Amino acid change and position (IMGT numbering) are indicated with lower case h for heavy chain and k for the kappa light chain.
