Top: formation efficiency (organoids per live cells) and size (organoid area) of AFOs at isolation (passage (P) 0) ( n = 26 independent AF samples for efficiency plot and n = 197 organoids for area plot; median and quartiles for both plots). Bottom: linear regression plot representing organoid formation efficiency (organoids per live cells) at various GAs. Color and size represent the total organoid number generated per sample; dashed line represents linear regression, R 2 = 0.05 and s.e.m. is shown in gray.
