Immunofluorescent staining showing AFO at P3 expressing the epithelial markers EpCAM, ECAD and pan-cytokeratin, while lacking expression of the mesenchymal marker PDGFRɑ. Immunofluorescent staining also shows AFO polarization, highlighted by the presence of the epithelial tight junction ZO-1 on the luminal surface and basolateral ITGβ4. Phalloidin counterstain highlights actin filaments (F-ACT) (scale bars, 50 m).
