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Experimental overview, myogenic (Mef2 + ) and neuronal (Elav + ) cells were isolated (>95% purity) from tightly staged embryos at 6-8 h and 10-12 h and used for Capture-C and ChIPseq (H3K27ac, CTCF, BEAF-32, Su(Hw)) in biological replicates. Gray = WE 2-3 h, red = myogenic mesoderm (myo 6-8 h, muscle 10-12 h), blue = nervous system (neuro 6-8 h, neurons 10-12 h). NM/NN (Mef2 - and Elav - cells).

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