Boxplots visualizing the change in mean expression (log-transformed counts) for immune checkpoint ligand PDL1, identified as significantly upregulated in HR-BR1 (LASP, LHS and macrophage) and HR-BR2 (LASP and macrophage) donors with respect to AR controls. FDR values derived from edgeR pseudobulk differential gene expression testing for AR versus HR-BR1 and AR versus HR-BR2 ( n = 33 in both comparisons; see Methods and Extended Data Fig. 4 for more details). The boxplot centers show median values while the minima/maxima show the 25th/75th percentiles, respectively, and whiskers extend to the most extreme datapoint within 1.5x interquartile range of the outer hinge of the boxplot.
