AlphaFold 3 (AF3) prediction of the NiV L active site in the presence of a duplex RNA, GTP, and two magnesium ions (green spheres). D722 (motif A) and D832 (motif C) are predicted to coordinate metals, and R511 (motif F) is positioned to interact with the phosphate of the incoming nucleotide. Template and nascent RNA are shown in dark and light gray, respectively. Parts of motifs A and D are semi-transparent for clarity. SeeFigure S2D for predicted local distance difference test (pLDDT) scores.
