UMAP of integrated space colored by Shannon’s entropy per spot and box plots of entropy, grouping spots by disease subtype. Box plots indicate the median (center lines), interquantile range (hinges) and 1.5 interquartile range (whiskers). n = 32,409 immune cell-enriched spots and 5,108 tumor cell-enriched spots. n = 47, 493, 467 and 74 in basal-, MBC-, LumA- and LumB-enriched spots. Two-sided independent two-sample t -test was performed on the entropy of each group comparison. P value = 7.89 10 -160 in comparison between immune cells and tumor cells; P values = 1.08 10 -2, 2.04 10 -142, 2.30 10 -52, 1.99 10 -49, 2.31 10 -7 and 2.14 10 -2 for basal versus MBC, MBC versus LumA, LumA versus LumB, basal versus LumA, MBC versus LumB and basal versus LumB. *** P < 0.001, **** P < 0.0001.
