Boxplot of the Z -scores for variants in the cDNA and prime editing screens ( P = 1.192 10 -6 ), considering only the most efficient pegRNA for each variant. Statistics for c and d shown for two-sided t -test with Bonferroni correction. * P 0.05; ** P 0.01; *** P 0.001; ****P 0.0001; NS, not significant ( P > 0.05). In all boxplots ( c and d ), boxes indicate the median and IQR for each sample with whiskers extending 1.5 IQR past the upper and lower quartiles. All Z -scores were calculated from the LFC of each pegRNA, in turn calculated using MAGeCK from the median values across n = 3 biologically independent samples.
