Scatter plot of sgRNA effect sizes as determined by different time point comparisons. Each dot shows the average of two biological replicates for an sgRNA. Black or colored dots are sgRNAs targeting the TSS or enhancers, respectively. The sgRNAs along the diagonal line of points, including sgTSS-1, drop out by T7 and thus are absent from the T7 versus T21 comparison. sgRNAs selected for validation assays are labeled.
