Distribution of average guide effects calculated from two experimental replicates for sgRNAs falling within peaks identified by different CRISPR screen analysis tools (center line, median; notch, confidence interval of the median; box limits, first and third quartiles; whiskers, range of all data points; violin, kernel density estimation; n =204, 1,218, 715, 623 and 71 sgRNAs within CREs from left to right; Welchs two-tailed t -test versus shuffled log 10 ( P )=55.2, 59.3, 68.8, 66.6 and 8.3).
