sgRNA-mediated growth effects (blue), H3K27ac ChIP signal (pink) and DHS (gray) for a CRISPRi growth screen at the GATA1 locus. sgRNAs were filtered to remove any low-specificity sgRNAs (GuideScan aggregated CFD < 0.2), which could cause confounding off-target toxicities. Dense tracks show peak calls using five different CRISPR screen analysis tools: CASA (orange), aggrDESeq2 (green), MAGeCK (red), CRISPR-SURF (purple) and RELICS (brown). Zoomed-in regions show log 2 (FC) of individual sgRNA effects (points indicate the mean values, and bars indicate the minimummaximum range of observations between n =2 replicates).
