guide dropout rate (dropout defined as less than ten mapped reads) in diverse CRISPRi screens with varying sequencing depth (bootstrap sampled). Dots show an average over 100 bootstrap samples. The GATA1 (circles) and MYC (triangles) screens in human K562 cells were performed with varied readout methods (colors). The GITR screen (rectangle) in mouse regulatory T cells (T reg ) used protein staining followed by sorting. The growth datasets included are (1) Tycko et al. 17 and (2) Fulco et al. 12 .
