Mean abundance of identified amino acids from the two peptides. Results from each independent experiment are shown in different colors. is the Spearman’s rank correlation coefficient, and P values were calculated from the Spearman’s rank correlation test. Hydrolysis and detection were performed in electrolyte buffer (1 M KCl, 10 mM MOPS, pH 7.5). Data are presented as mean ± s.d. n = 3 independent experiments.