Left: representative images of the same MyD88-GFP cluster at 0 and 30 min following UV illumination. Scale bar, 2 µm. Right: quantification of relative change in MyD88-GFP cluster size 30 min following UV illumination in MyD88 clusters without IRAK4 recruitment (0.78 ± 0.05, n = 5) and in MyD88 clusters with IRAK4 recruitment (0.61 ± 0.10, n = 10). Data presented as mean ± s.d. Significance was tested using the two-tailed unpaired t -test (** P = 0.003). N = 3. Center line in the box plot represents the median, the box represents 25–75% of the data, whiskers represent 1.5 × interquartile range and the square box among data points represents the mean.
