TIRF images (top) and flow cytometry results (bottom) of HeLa cells expressing mScarlet-CD4-PhoCl-RER with antibodies against mScarlet following varying doses of UV illumination. N = 3. Data presented as mean ± s.d. Center line in the box plot represents the median, the box represents 25–75% of the data, whiskers represent 1.5 × interquartile range and the square box among data points represents the mean. Scale bars, 10 µm.
