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Schematic of the experimental procedure. Sequential excitation at 940 nm and 1,040 nm was used for dual-color imaging of cAMPinG1-ST and RCaMP3. Three optical planes spaced 30 m apart were imaged at 3.4 Hz per plane using a piezo objective scanner.
#Workflow#Illustration#Experimental Procedure#Excitation#Dual-color Imaging#cAMPinG1-ST#RCaMP3#Optical Planes#Piezo Objective Scanner
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