Quantification of axon split and merge error rate for randomly seeded axons, evaluated on various 3D-EM data: human cortex multiSEM dataset 18 (crosses) (two agglomeration states analyzed (c2,c3) with 8% split resolution from c3 to c2); mouse cortex multiSEM dataset (Si150L4, https://wklink.org/7122 ); mouse cortex SBEM dataset 1 . Purple symbols show the effect of applying RoboEM to previous reconstruction. The red dashed line denotes the same merge-rate comparison for human data (3.5-fold improved split resolution) and mouse data (3.1-fold improved split resolution). Purple lines denote RoboEM split-rate improvement with modest merge rate increases yielding error rates tolerable for more complex connectomic analyses (Fig. 1f ). The black dashed line denotes a human annotator-based error resolution (as published in ref. 1 ) shown for comparison.
