Standard workflow for connectomic analyses from 3D-EM data: initial volume segmentation (two-step process of classification and watershed 17 , 22 , 24 , 25 , 26 , 27 , 28 , or directly as foreground classification 30 , 31 , 32 ); automated agglomeration (based on interfaces between segments); manual inspection to resolve remaining errors and reach reconstruction quality usable for meaningful connectomic analysis 1 , 2 , 12 , 16 , 19 , 37 , 38 , 39 , 40 , 41 . Data cubes with 10 m edge lengths are shown.
