IMs of WT and Ffar2/Ffar3 -/- mice were isolated from the bone marrow by FACS sorting, stimulated for 20 minutes with 1 mM acetate (Ace) or control medium prior to incubation with K. pneumoniae. WT IMs were also stimulated with 1 mM acetate in combination with 50 M bafilomycin A1 (Baf), 0.75 g cytochalasin D (Cyto), or 100 g N,N´,N”-Triacetylchitotriose (Tri) prior to incubation with the bacteria. Values are shown as median, each dot represents the mean data from triplicates from cells of one mouse ( n = 8 for K. pneumoniae without IMs; n = 12 for IM WT ; n = 12 for IM WT + Ace; n = 9 for IM WT + Ace + Baf; n = 9 IM WT + Ace + Cyto; n = 9 for IM WT + Ace + Tri; n = 12 for IMFfar2/Ffar3-/- ; n = 12 for IM Ffar2/Ffar3 -/- + Ace). Kruskal-Wallis test followed by Dunn's multiple comparison was applied to the dataset.
