Free energy profiles for HIV-1 capsid passage through the NPC as a function of the vertical distance zC−zibetween the capsid center, halfway between its narrow and wide ends, and the inner ring (seeFigures S7A–S7C for the capsid positions used to calculate the mean force shown inFigure S7D). Black dashed lines mark the extent of the NPC, and the vertical red dashed line indicates the position of the steric blockage of the HIV-1 capsid inside the intact NPC.
