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CRISPR design and experiment strategy to target NFKB2 expression. The guide RNA (gRNA) targets the Rel homology domain (RHD) of NFKB2 introducing a nonsense mutation. KMS-11 cells are transduced with CRISPR targeting plasmids, induced with doxycycline (Dox) for Cas9 expression and sorted by flow cytometry to harvest Cas9/gRNA-expressing clones for downstream next-generation-sequencing (NGS) experiments.

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