MDMs were infected with NNHIV for 24 h (upper) or 48 h (lower) and stained for HIV-1 CA (green) and lamin A/C (magenta). Shown are confocal z slices, and dashed lines mark cell boundaries. Enlarged regions (center, right) display HIV-1 CA signals (white arrowheads) accumulated at the NE as defined by lamin A/C staining. Scale bars: 5 μm (overviews), 1 μm (enlargements).