Gel from Scientific Research

Western blot to evaluate the expression levels of RAD51 mutants, with alpha-tubulin as a loading control. The membrane was separated at a 50-kDa line (Supplementary Fig. 2a) and proteins were detected by each antibody. The uncropped membrane scans are shown in Supplementary Fig. 2a. Reproducibility was confirmed by three independent experiments. WT, wild type.

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