CD45.1 congenic P14 T cells were transferred into C57BL/6 host mice that were infected with LCMV Armstrong. PD-1 hi and PD-1 lo P14 cells (gating strategy shown in Extended Data Fig. 3b ) were collected at 4.5 days post-infection (dpi) and subsequently retransferred into naive C57BL/6 host mice that were infected with LCMV Armstrong on the same day of P14 transfer. Retransferred P14 cells from PD-1 hi versus PD-1 lo recipients were collected at 15 days after infection and sent for single-cell sequencing. Graphics were created with Biorender.com.
