The human HPRT1 locus was cloned into a assemblon vector and flanked by lox recombination sites for Big-IN integration. HPRT1R was assembled de novo from 28 synthetic segments, shown below the locus. Vector components include centromere (CEN)autonomously replicating sequence (ARS) and LEU2 for propagation and selection in S. cerevisiae , bacterial artificial chromosome (BAC) oriS and oriV (low copy and inducible high copy origins, respectively) and the kanamycin resistance gene (Kan r ) for propagation and selection in Escherichia coli , and eGFPT2ABSD for transient selection in mammalian cells. Chr., chromosome.
