Stacked Bar Plot from Scientific Research

Coupling between the neuronal membrane and the actin cytoskeleton is required for cortical neurons to respond to mechanical stimuli. Bar plots give the percentage of cortical neurons showing a response (grey) and no response (white) to mechanical stimulation of untreated (control) neurons and of neurons treated with 0.1 μM latrunculin A (LatA) or 50 μM NSC668394C. The neurons were indented with a 5-μm-diameter silica bead by applying a force of 200 nN ( ~ 5 kPa) at 10 μm s –1 . The neurons expressed the genetically encoded calcium sensor GCaMP6s. The response of the neurons expressing GCaMP6s was monitored by confocal microscopy. In all experiments, spontaneous activity of the neurons was blocked with glutamate receptor antagonists (10 μM DNQX and 40 μM D-AP5) to isolate mechanically evoked responses. n represents the number of characterized independent neurons. P values of the χ 2 analysis for different conditions are given above the bars in c.

DOI