Scatter Plot from Scientific Research

Quantification of mitotic cells via immunocytochemistry. U2OS cells were treated with DMSO (negative control), F5, nocodazole (noc, positive control) or colchicine (col, positive control) for 24 h before staining of cells for phospho-histone H3 and DNA. Cells in mitosis were quantified as the percentage of phospho-histone H3-positive cells. Data are mean values s.d. of three independent replicates ( n = 3). Statistical analyses were performed relative to the DMSO control by employing unpaired two-tailed t-tests (**** P < 0.0001). The P values relative to cells treated with DMSO are <0.0001 for F5 (10 M)-treated, <0.0001 for F5 (30 M)-treated, <0.0001 for noc-treated and <0.0001 for col-treated cells.

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