Selected compounds with structural variation to the most active compound ( A23 ) in an osteoblast differentiation assay. Cell viability was assessed using a CellTiter-Glo Luminescent Cell Viability Assay and treating C3H10T1/2 cells with compound (30 M) in the absence of purmorphamine for 96 h. The viability of cells treated with DMSO was set to 100%. IC 50 , half-maximal inhibitory concentration.
