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Alpl gene expression; C3H10T1/2 cells were incubated for 96 h with 1.5 M purmorphamine and DMSO, 1 M vismodegib (vismo) or 1, 5 or 10 M of A23 before RTqPCR. Data are mean values s.d. of three biological replicates ( n = 3). The P values relative to cells treated with DMSO and purmorphamine are <0.0001 for DMSO-treated, <0.0001 for vismo (1 M)-treated, 0.0014 for A23 (1 M)-treated, 0.0005 for A23 (5 M)-treated and 0.0006 for A23 (10 M)-treated cells.

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