The frequency distribution of the normalized CTC distance between condensates (FUS N FUS N , n = 93; FUS N DDX4 N , n = 113). Normalization is obtained by dividing the measured distance between condensates with the sum of the radii of the two condensates. The perfect colocalization is plotted by setting the frequency to 100% for the lowest bin (black dashed line; Methods for details). The normalized CTC distance is approximately equal to 0 for two miscible condensates and is approximately equal to 1 for two associated immiscible condensates. The IDR1 (24 mer)IDR2 (60 mer) represents respective fusion of the two IDRs to the cores. For example, FUS N DDX4 N indicates the FUS N is fused to 24-mer ferritin core and DDX4 N is fused to 60-mer I3-01 K129A core. All measurements are independent.
