Schematics of the optogenetic Corelet experiment in living cells showing oligomerization-driven demixing from the nucleolus. At low valence, the NPM1mCherrySspB remains within the nucleolus (orange phase). As valence is increased, there is first an interfacial enrichment of NPM1, followed by formation of de novo droplets (green phase). Higher valence implies a greater fraction of NPM1mCherrySspB is bound to the ferritin core.
