Two-photon microscopy images of cleared pLNs from mTmG mice at 24 h post-footpad LCMV infection (full 3D stack inVideo S1). Mice were injected with CTV+ WT B cells and DR+ EBI2 KO B cells 90 min and anti-CD45.2-AF488 (green) 2 min prior to harvest. HEV contour shown in magenta. Image is representative of 4 LNs across 2 mice and 2 independent experiments. Scale bars, 30 μm (top-down image) or 40 μm (side view images).
