Workflow from Scientific Research

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Design of mutagenesis MPRA. Each original 400 bp enhancer was divided into three 171 bp subregions (F1–F3), and each subregion was tiled with 10 bp deletions. The barcoded oligos were inserted into a lentiMPRA vector so that the barcode was in the reporter gene’s 3′ UTR.
#Workflow#Flowchart#Illustration#Mutagenesis MPRA#Enhancer#Subregions#Barcoded Oligos#LentiMPRA Vector#Reporter Gene
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