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LentiSTARR-seq validation. Seventeen active and seven inactive enhancers neighboring cardiovascular disease genes were cloned individually into the lentiMPRA vector. iPSC-CMs were transduced on day 17 and assayed on day 24. GFP fluorescence of the empty vector control and enhancer-reporter lentiviruses was measured by flow cytometry. The numbers above bars show a number of independent biological replicates. Numbers at the top show one-sided t -test for activity above empty vector with BenjaminiHochberg multiple testing correction. Blue indicates P adj < 0.05.
#Bar Plot#Error Bars#Scatter Plot#LentiSTARR-seq#Validation#Active Enhancers#Inactive Enhancers#Cardiovascular Disease Genes#lentiMPRA Vector#iPSC-CMs#GFP Fluorescence#Flow Cytometry#t-test#Benjamini-Hochberg Correction
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