LentiSTARR-seq validation. Seventeen active and seven inactive enhancers neighboring cardiovascular disease genes were cloned individually into the lentiMPRA vector. iPSC-CMs were transduced on day 17 and assayed on day 24. GFP fluorescence of the empty vector control and enhancer-reporter lentiviruses was measured by flow cytometry. The numbers above bars show a number of independent biological replicates. Numbers at the top show one-sided t -test for activity above empty vector with BenjaminiHochberg multiple testing correction. Blue indicates P adj < 0.05.
