Heatmap from Scientific Research

We applied SATURN to regroup cell types in a multispecies context. By clustering SATURN’s embeddings, we found five broad cell types. Heat map and dendrogram of reannotated cell types using SATURN. Labels on the right side show original cell-type annotations, while on the bottom we show reannotations obtained using SATURN. These clusters include cell types originally labeled as fibroblast and beam A/Y cells (cluster 1), beam A and uveal cells (cluster 2), JCT and beam cells (cluster 3 and cluster 4) and corneal endothelium cells (cluster 5). Across 30 independent experiments, we regrouped cluster 1 as fibroblast cells, cluster 2 as beam A cells, clusters 3 and 4 as JCT cells, and cluster 5 as corneal endothelium cells. We specifically reannotated mouse beam A and beam Y cells, which have high expression of fibroblast markers such as Pi16 , Fbn1 and Mfap5 as originally noted 18 . We additionally regrouped human beam B cells, which were not found in other species, as JCT cells. Finally, we mapped beam X cells, which were unique to rhesus and cynomolgus macaque, to two JCT clusters.

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