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Schematic diagram of the optical set-up used for multi-sheet RESOLFT imaging. AM, alignment module; BS, beam splitter; CAM1, oblique detection camera; CAM2, orthogonal detection camera; CYL, cylindrical lens; DG, diffraction grid; D N , dichroic mirror; FM, flip mirror; GM, galvanometric mirror; GT, Glan-Thompson polarizer; I N , iris; M N , mirror; MS, motorized stage; O N , objective; RR, remote refocus module; SM, scanning module; TL N , tube lens. The inset on the left shows the illumination pattern intensities probed with a fluorescent bead and reconstructed.

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