The thickness of the confined sheets depends on the time and intensity of the off-switching illumination. The top graph shows color-coded cross-sectional intensity profiles of the off-switching and read-out illuminations and the predicted confinement at 50 W cm -2 of off-switching intensity for different times with rsEGFP2 labeling. The bottom graph shows the expected thickness of each emitting sheet at increased off-switching times. FWHM, full width at half-maximum.
