Volcano plot of the two-sided two-sample t -test comparison of 28S rRNA digested versus undigested TREX samples ( n = 5 biological replicates), showing the enrichment of RPL proteins, known large subunit RAFs and known large subunit processing RBFs, as well as RPS11, RPS15, RPS19, RPS25 and RPS26. Curved lines in all volcano plots mark the significance boundary (FDR = 0.05, S0 = 0.1).