Microscopy from Scientific Research

HEK293 cells coexpressing mTq2-tagged WT rhodopsin and either YFP-tagged P23H or G188R rhodopsin. Each column shows images of fluorescence from YFP-tagged mutant rhodopsin (red), mTq2-tagged WT rhodopsin (green), overlays of the fluorescence from YFP-tagged mutant rhodopsin and mTq2-tagged WT rhodopsin (yellow), PM marker (blue), overlays of fluorescence from YFP-tagged mutant rhodopsin and the PM marker (magenta), and overlays of fluorescence from mTq2-tagged WT rhodopsin and the PM marker (cyan). Nuclei were stained by DAPI (blue) and were shown in all images except the last 3 columns in B . Scale bar, 5 m. Images are representative of at least three different experiments.

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