The representative images show the distribution of CTTNBP2-GFP (green) in NSCs after 24 h of teleofection. Nuclei and F-actin were labeled by DAPI and phalloidin in blue and red, respectively. The colocalization of CTTNBP2-GFP and F-actin was manifested as yellow. The orthogonal view of white squares from NSCs provides a comprehensive understanding of the relative distribution of CTTNBP2-GFP and F-actin from multiple angles.