The flowchart shows the experiment design for serial transfection of indicated plasmids. The examples of images show the expression pattern of transfected neurons with GFP (green) or mCherry (red), and neurons were outlined by MAP2 (magenta). Under serial transfection, different nucleic acids can be uptaken by different neurons sequentially. The representative images show the neurons transfected with GFP followed by mCherry transfection at DIV10 and 11, respectively. The arrows indicate GFP and mCherry double positive neurons.
