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Whole-cell patch clamp recordings were made from CA1 pyramidal neurons in acute mouse hippocampal brain slices. Representative examples of standard current-voltage responses for slices incubated in soluble aliquots of the recombinant tau peptides (N terminus, n = 12; STA core, n = 12; fibril core, n = 12; or C terminus, n = 12) or control aCSF ( n = 12) for 1 h before electrophysiological recordings. Incubation with the STA core region peptide depolarized the RMP and increased the IR of the recorded neurons. Each ‘ n ’ in d – h represents a whole-cell patch clamp recording made from individual acute hippocampal brain slices incubated with one of the recombinant tau peptides (diluted in aCSF; Methods ) or control aCSF.
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