Log 2 -normalized counts of bulk RNA-seq with paired PD-1 hi and PD-1 lo P14 samples from the same donor mouse. Significance values were calculated using empirical Bayes moderated t -statistics and were adjusted with BenjaminiHochberg correction. n = 5 for all groups; lines represent mean and symbols represent individual mice. Box plots show median, first and third quartiles (hinges); lines show the smallest or largest observation within a distance from the nearest hinge of 1.5 times the size of the box. Observations outside this range are shown as outliers.